
Conditions

Column : Inertsil ODS-3V ( 5 m , 150 4.6mmI.D.)
Flow rate : 1.0mL/min
Eluent : Acetonitrile / Water = 50 / 50
Oven Temp. : 40
Detector : UV 220 nm
Samples : Estriol (1mg/mL)
: -Estradiol (1mg/mL)
: Ethynylestradiol (1mg/mL)
: Estrone (1mg/mL)
: Diethylstilbestrol (0.5mg/mL)
: Hexestrol (0.5mg/mL)
Sample Volume : 1 L
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