Analysis of Estradiols DATA NO. H13

Conditions

Column : Inertsil ODS-3V ( 5m , 1504.6mmI.D.)
Flow rate : 1.0mL/min
Eluent : CH3CN / 50mM KH2PO4(pH3.0 adjusted by H3PO4) = 45/55
Oven Temp. : 40
Detector : ECD 850mV vs Ag/AgCl Res.3.0 1000nA/V
Samples : -Estradiol
: -Estradiol
: Diethylstilbestrol
(each 500ppb in CH3CN/H2O=45/55)
Sample Volume : 5L
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