
Conditions

Column : Inertsil ODS-3V ( 5 m , 150 2.1mmI.D.)
Flow rate : 0.2mL/min
Eluent : CH3CN / 0.2% H3PO4 solution = 40/60
Oven Temp. : 40
Detector : ECD 950mV vs Ag/AgCl
Samples : Human Serum spiked at 10ng/mL of bisphenol A
 (SPE Conditions)
 Empore Disk cartridge SDB-RPS 4mm/1mL
 Activation : Acetonitrile 100 L , Condition : 0.2% phosphoric acid 300 L
 Load Sample : 200 L , Wash : 0.2% phosphoric acid 100 L
 Elute : Mobile Phase 50 L twice
Sample Volume : 20 L
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