Conditions
 Column : Inertsil WP300 C8 ( 5 m , 150 4.6mmI.D.)
Flow rate : 1mL/min
|
Eluent : |
A: water (0.1% TFA) |
| B: Acetonitrile:water=90:10 (0.1% TFA) |
Gradient : A/B 80/20 _ 25min - 40/60
Oven Temp. : 30
Detector : UV 280 nm
|
Samples : |
1.Ribonuclease (0.125 mg/mL) |
| 2.Insulin (0.04mg/mL) |
| 3.Cytochrome (0.1 mg/mL) |
| 4.Lysozyme (0.129 mg/mL) |
| 5.BSA (0.26 mg/mL) |
| 6.Trypsin Inhibitor from Soybean (0.25 mg/mL) |
| 7.Ovalbmin (0.25 mg/mL) |
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