Conditions
 Column : Bioptic AV-1 ( 5 m , 150 4.6mmI.D.)
Flow rate : 1.0mL/min
Eluent : Methanol /50mM phosphate buffer (pH6.6) = 8 / 92 (w / w)
Detector : UV 340nm
Oven Temp. : 40
|
Sample : |
10uL Amino acid sol.(10mg/mL 50%MeOH)+ 40uL OPA sol. |
| L 2-mercaptoethanol in 10mL borate buffer pH9.2) |
| mixing |
Sample Volume : 5L
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